Estimation of Total Phenolic Compounds In Olive Leaves As Biomarker For Assessing The Lead Pollution At Banias Area.

Authors

  • Kamel Khalil
  • Chahid Moustapha
  • Abeer Mahfoud

Abstract

 

The aim of this study was to estimate the total amount of phenolic compounds of olive trees leaves (Olea europaea L.) as Biomarker for the assessment of lead air pollution at Banias area. Olive trees were selected as the predominant species in the study area. The samples were collected from 6 locations at different distances from the vicinity of the Banias refinery (0.1, 0.5, 2, 4, 6, 10) Km. The dominated wind in the region is western - southwesterly during the winter (March) and summer (September), The control was taken from an area about 20 km from the refinery to the north-east (al-Qardahah rural).

The concentration of total phenolic compounds (TPC) during the summer was (45.6-70.85) mg GAE/g dw and in the control (40.4) mg GAE/g dw, and during the winter the content of TPC was (35.6-52.9) mg GAE/g dw and in the control (25.8) mg GAE/g dw. The concentration of Lead (Pb) in unwashed leaves during the summer was (0.879 – 2.170) ppm and in the control (0.005) ppm, whilst during the winter was (0.479-1.023) ppm and in the control (0.00083) ppm. The results showed significant differences in the concentration of total phenolic compounds and lead between sites (Anova test). High concentration of total phenols and lead in the summer compared to values in winter at all sites (t-test). The results also showed a negative correlation between the concentration of total phenolic compounds and lead with the distance from the refinery of Banias. Finally, The results showed that the TPC can be used as biomarker of lead air pollution in Banias area.

References

• إبراهيم، بهجت. دراسة بعض خصائص الرياح والأمواج البحرية على شاطئ مدينة طرطوس (سورية). مجلة جامعة تشرين للبحوث والدراسات العلمية، 30 ,2008، 9-21.

• إبراهيم، دينا. دراسة إمكانية مراكمة بعض الأنواع المزروعة في حرم مصفاة بانياس للعناصر الثقيلة. رسالة ماجستير في كلية الزراعة جامعة تشرين، (2014)، 60 صفحة.

• AlQUTOB, M.A; SHQAIR, H; MALASSA, H; DAVIS, J-M; Al-RIMAWI, F. Determination of trace metals in harvested rain water after the November2012 bombing in Gaza by using ICP/MS. Journal of Materials and Environmental Science. 7(9), 2016, 3477-3488.

• ALLEN, S. E. Analysis of Ecological Materials. 2nd ed., Oxford: Blackwell Scientific Publication. 1989, 380 pages.

• ANZAR, J; RICHER, M; BEGIN, C; BEGIN, Y. Lead Exclusion and Copper Translocation in Black Spruce Needles. Springer, Water Air Soil Pollut. 203, 2009, 139-

• AINSWORTH, E; GILLESPIE, K. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. NATURE PROTOCOLS. 2(4), 2007, 875-877.

• AL-DABBAS, M; ALI, L; AFAJ, A. The effect of Kirkuk Oil Refinery on Air pollution of Kirkuk City-Iraq. Iraqi Journal of Science, Proceeding of the 1st Conference on Dust Storms and their environmental effects. 17-18 Oct, 2012.

• AL-DABBAS, M; ALI, L; AFAJ, A. The chemistry of the leaves of plant Eucalyptus camaldulensis as environmental contamination indicator of selected locations at Kirkuk – Iraq. Bull Iraq nat Hist Mus. 13(2), 2015, 39-50.

• ALONSO-HERNANDEZ, C.M; BERNAL-CASTILLO, J; BOLANOS-ALVAREZ, Y; GOMEZ-BATISTA, M; DIAZ-ASENCIO, M. Heavy metal content of bottom ashes from a fuel oil power plant and oil refinery in Cuba. Fuel. 90, 2011, 2820-2823.

• AZAD NOORANI, H.; SHIVA, A.H; MALEKPOUR, R. 2011. Toxic Effects of Lead on Growth and Some Biochemical and Ionic Parameters of Sunflower (Helianthus annuus L.) Seedlings. Current Research Journal of Biological Sciences. 3(4), 398-403.

• BRAHMI, F; MECHRI, B; DABBOU, S; DHIBI, M; HAMMAMI, M. The efficacy of phenolics compounds with different polarities as antioxidants from olive leaves depending on seasonal variations. Industrial Crops and Products. 38, 2012, 146-152.

• BURZYNSKI, M; KLOBUS, G. Changes of photosynthetic parameters in cucumber leaves under Cu, Cd, and Pb stress. Photosynthetica. 42(4), 2004, 505-510.

• BALASOORIYAA, B.L.W.K; SAMSON, R; MBIKWA, F; VITHARANA, U.W.A; BOECKX, P; VAN, M. Biomonitoring of urban habitat quality by anatomical and chemicalleaf characteristics. ELSEVIER, Environment and Experimental Botany. 65, 2009, 386-394.

• BACELAR, E; SANTOS, D; MOUTINHO, J; GONCALVES, B; FERREIRA, H; CORREIA, C. Immediate responses and adaptative strategies of three olive cultivars under contrasting water availability regimes: Changes on structure and chemical composition of foliage and oxidative damage. ELSEVIER, Plant Science. 170, 2006, 596-605.

• CHAROENPRASERT, S; MITCHELL, A. Factors Influencing Phenolic Compounds in Table Olives(Olea europaea). Journal of Agricultural and Food Chemistry. 60, 2012, 7081-7095.

• DEEPIKA, C; VERMA, P.U; SOLANKI, H.A; PATEL, Y.M. Role of tolat phenol in the resistance mechanism of plants against air pollution. 2(2), 2014, 586-592.

• FURLAN, C; SALATINO, A; DOMINGOS, M. Leaf contents of nitrogen and phenolic compounds and their bearing with the herbivore damage to Tibouchina pulchra Cogn. (Melastomataceae), under the influence of air pollutants from industries of Cubatão, São Paulo. Revta brasil. Bot., São Paulo. 22(2), 1999, 317-323.

• GHADERIAN, S.M; HEMMAT, G.R; REEVES, R.D; BAKER, A.J.M. Accumulation of lead and zinc by plants colonizing a metal mining area in Central Iran. Journal of applied Botany and Food Quality. 81, 2007, 145-150.

• GHELICH, S; ZARINKAMAR, F; NIKNAM, V. Determination of peroxidase activity, total phenolic and flavonoid compounds due to Lead toxicity in Medicago sativa L. Advances in Environmental Biology. 6(8), 2012, 2357-2364.

• HASSANIEN, M. Atmospheric Heavy Metals Pollution: Exposure And Prevention Policies In Mediterranean Basin. Springer, Environmental Heavy Metal Pollution and Effects on Child Mental Development: Risk Assessment and Prevention Strategies. 2011, 287-307.

• HABIB, H; AWADH, S; MUSLIM, M. Toxic heavy metals in soil and some plants in Baghdad, Iraq. Journal of Al-Nahrain University. 15(2), 2012, 1-16.

• HAMEED, N; SIDDIQUI, Z; AHMED, S. Effects of Copper and Lead on Germination, Accumulation and Phenolic Contents of Spinancea oleracea and Lycopersicum esculentum. Pakistan Journal of Biological Sciences. 4(7), 2001, 809-811.

• HSEU, Z. Evaluating heavy metal contents in nine composts usingfour digestion methods. ELSEVIER, Bioresource Technology. 95, 2004, 53-59.

• HU, Y; WANG, D; WEI, L; ZHANG, X; SONG, B. Bioaccumulation of heavy metals in plant leaves from Yan'an city of the Loess Plateau, China. Ecotoxicology and Environmental Safety. 10, 2014, 82-88.

• KABATA-PENDIAS, A; PENDIAS, H. Trace elements in soil and plants. 3rd Edition, CRC Press Boca Raton. 2001, 403 pages.

• KAIMOYO, E; FARAG, M; SUMNER, L; WASMANN, C; CUELLO, J; VANETTEN, H. Sub-lethal levels of electric current elicit the biosynthesis of plant secondary metabolites. Biotechnol. 24, 2008, 377-384.

• KASTORI, R; PLESNICAR, M; SAKAC, Z; ARSENIJEVIC-MAKSIMOVIC, I. Effect of excess lead on sunflower growth and photosynthesis. Journal of Plant Nutrition. 21(1), 1998, 75-85.

• KHATTAK, M. I; JABEEN, R. Detection of heavy metals in leaves of Melia azedarach and Eucalyptus Citriodora az biomonitoring tools in the region of Quetta valley. Pakistan Journal of Botany. 44(2), 2012, 675-681.

• KLUMPP, A; KLUMPP, G; DOMINGOA, M. Plants as bioindicators of air pollution at Theserra Do Mar near the industrial complex of Cubatao, Brazil. Environmental Pollution. 85, 1994, 109-116.

• KOVACIK, J; BACKOR, M. Phenylalanine Ammonia-Lyase and Phenolic Compoundsin Chamomile Tolerance to Cadmium and Copper Excess. Springer, Water Air Soil Pollut. 185, 2007, 185-193.

• KISA, D; ELMASTAS, M; OZTURK, L. Responses of the phenolic compounds of Zea mays under heavymetal stress. Springer, Appl Biol Chem .59(6), 2016, 813-820.

• LATTANZIO, V; LATTANZIO, V.M.T; CARDINALI, A. Role of phenolics in theresistance mechanisms ofplants against fungalpathogens and insects. Research Signpost, Phytochemistry: Advances in Research. 2006, 23-67.

• MANSOUR R. The pollution of tree leaves with heavy metal in Syria. International Journal of ChemTech Research. 6(4), 2014, 2283-2290.

• MCCARTY, L.S; MUNKITTRICK, K.R. Bioindicators Versus Biomarkers in Ecological Risk Assessment. Human and Ecological Risk Assessment. 8(1), 2002, 159-164.

• MICHALAK, A. Phenolic Compounds and Their Antioxidant Activity in Plants Growing under Heavy Metal Stress. Polish J. of Environ. Stud. 15(4), 2006, 523-530.

• MUSZYNSKA, E; KALNZNY, K; HANUS, E. Phenolic compounds in Hippophae Rhamnoides leaves collected from heavy metals contaminated sites. Plants in Urban Areas and Landscape. 2014, 11-14.

• NAJAFI, S; JAMEI, R. Effect of Silver Nanoparticles and Pb(NO3)2 on the Yield and Chemical Composition of Mung bean(Vigna radiata). Journal of Stress Physiology & Biochemistry. 10(1), 2014, 316-325.

• NOROUZI, S; KHADEMI, H. Source identification of heavy metals in atmospheric dust using Platanus orientalis L. leaves as bioindicator. Eurasian Journal of Soil Science. 4(3), 2015, 144-152.

• OLIVA, S; MINGORANCE, M.D. Assessment of airborne heavy metal pollution by above ground plant parts. ELSEVIER, Chemosphere. 65, 2006, 177-182.

• OLIVARES, E. The effect of lead on the phytochemistry of Tithonia diversifolia exposed to roadside automotive pollution or grown in pots of Pb-supplemented soil. Braz. J. Plant Physiol. 15(3), 149-158, 2003

• PAZOKI, A. Evaluation of flavonoids and phenols content of wheat under different Lead, PGPR and Mycorrhiza levels. Biological forum. 7(1), 2015, 309-315.

• PERALBO-MOLINA, A; LUQUE DE CASTRO, M.D. Potential of residues from the Mediterranean agriculture and agrifood industry. Trends in Food science & Technology. 2013, 1-9.

• PASQUALINI, V; ROBLES, C; GARZINO, S; GREFF, S; BOUSQUET, A; BONIN, G. Phenolic compounds content in Pinus halepensis Mill. needles:a bioindicator of air pollution. Chemosphere. 52, 2003, 239-248.

• PIETTA, P.G. Flavonoids as Antioxidants. J. Nat. Prod. 63, 2000, 1035-1042.

• PAIS, I; JONES, B. The handbook of trace elements.1997, 240 pages.

• RISTIC, M; PERIC, A; ANTANASIJEVIC, D; RISTIC, M; UROSEVIC, M; TOMASEVIC, M. Plants as Monitors of Lead Air Pollution. Pollutant Diseases, Remediation and Recycling. 2013, 387-431.

• REDDY, A.M; KUMAR, S.G; JYOTSNAKUMARI, G; THIMMANAYAK, S; SUDHAKAR, C. Lead induced changes in antioxidant metabolism of horsegram (Macrotyloma uniflorum (Lam.) Verdc.) and bengalgram (Cicer arietinum L.). Chemosph. 2005, 60, 97–104.

• SALIH, A.A; MOHAMED, A.A; ABAHUSSAIN, A.A; TASHTOOS, F. Use of Some Trees to Mitigate Air and Soil Pollution Around Oil Refinery, Kingdom of Bahrain. Journal of Environmental Science and Pollution Research. 3(2), 2017, 167–170.

• SAWIDIS, T; BREUSTE, J; MITROVIC, M; PAVLOVIC, P; TSIGARIDAS. Trees as bioindicator of heavy metal pollution in three European cities. Environmental Pollution. 159, 2011, 3560-3570.

• SHARMA, P; DUBEY, R. Lead toxicity in plant. Plant physiology. 17, 2005, 32-52.

• SHARMA, R.K; AGRAWAL, M; AGRAWAL, S.B. Physiological, biochemical and growth responses of Lady’s Finger (Abelmoschus esculentus L.) plants as affected by Cd contaminated soil. Bull Environ Contam Toxicol. 84, 2010, 765-770.

• STANKOVIC, M. Total phenolic content, flavonid concentration and antioxidant activity of Marrubium peregrinum L. Extracts. Kragujevac J. Sci. 33, 2011, 63-72.

• STANCHEVA, I; GENEVA, M; MARKOVSKA, Y; TZVETKOVA, N; MITOVA, I; TODOROVA, M; PETROV, P. A comparative study on plant morphology, gas exchange parameters, and antioxidant response of Ocimum basilicum L. and Origanum vulgare L. grown on industrially polluted soil. Turkish Journal of Biology. 38, 2014, 89-102.

• SINGLETON, V; ORTHOFER, R; LAMUELA, R.M. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods In Enzymology. 299, 1999, 152-178.

• SIATKA, T; KASPAROVA, M. Seasonal Variation in Total Phenolic and Flavonoid Contents and DPPH Scavenging Activity of Bellis perennis L. Flowers. Molecules. 15, 2010, 9450-9461.

• TURAN, D; KOCAHAKIMOGLU, C; KAVCAR, P; GAYGISIZ, H; ATATANIR, L; TURGUT, C; SOFUOGLU, S. The use of olive tree (Olea europaea L.) leaves as a bioindicator for environmental pollution in the Province of Aydin, Turkey. Environ Sci Pollut Res. 18, 2011, 355-364.

• TALHAOUI, N; TAAMALLI, A; GOMEZ, A.M; FERNANDEZ, A; SEGURA, A. Phenolic compounds in olive leaves: Analytical determination, biotic and abiotic influence, and health benefits. Food Research International. 77, 2, 2015, 92-108.

• UDOSEN, E.D; UWAH, E.I; JONATHAN, I.I. Levels of trace metals in washed and unwashed leaves of roadsides Vernonia amygdalina obtained in Abak, Akwalbom state, Nigeria. International Journal of Advances in Pharmacy, Biology and Chemistry. 6(2), 2017, 131-138.

• ZHELJAZKOV, V; NIELSEN, N. Effect of heavy metals on peppermint and cornmint. Plant and Soil. 178, 1996, 59-66.

• ZHENG, W; WANG, S. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem. 49(11), 2001, 5165-5170.

• ZOBEL, A; NIGHSWANDER, J. Accumulation of phenolic compounds in the necrotic areas of Austrian and red pine needles after spraying with sulphuric acid: a possible bioindicator of air pollution. New Phytol. 117, 1991, 565-574.

• ZHOU, Z; HUANG, S; GUO, K; MEHTA, S; ZHANG, P; YANG, Z. Metabolic adaptations to mercury-induced oxidative stress in roots of Medicago sativa L. Journal of Inorganic Biochemistry. 101, 2007, 1-9.

• <https://ar.climate-data.org>[(accessed on 20 October 2016)].

• <https://earth.google.com/web/>[(accessed on 1 November 2017)].

• <https://www.google.com/maps/@35.2159007,35.9733386,12.54z>[(accessed on 5 March 2018)].

• <http://www.thaweaonline.sy>[(accessed on 19 August 2016)].

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Published

2018-12-24

How to Cite

1.
Khalil K, Moustapha C, Mahfoud A. Estimation of Total Phenolic Compounds In Olive Leaves As Biomarker For Assessing The Lead Pollution At Banias Area. TUJ-BA [Internet]. 2018Dec.24 [cited 2024Mar.28];40(6). Available from: https://journal.tishreen.edu.sy/index.php/bassnc/article/view/5715