The identification and detection of curcumin and its constituent compounds using high-performance liquid chromatography (HPLC) technology, and determination of the optimal conditions for the developed analytical method
Abstract
This study aimed to develop a sensitive and accurate analytical method to determine and
detect curcumin using high-performance liquid chromatography HPLC. Optimal conditions such as mobile phase, temperature, flow speed, and the maximum wavelength for analysis λmax were studied. The study was carried out on a standard solution of curcumin with a concentration of (40ppm) and a separation column (C18) was used with specifications (250 ×4.6m.m.5μm).
The results showed the optimal conditions were as follows: the flow speed of the mobile
phase was (0.8 ml/min) as a mixture of acetonitrile and phosphoric acid (0.1%) in a ratio of (40:60 v/v), the temperature of the separation column was (40 ̊C), while λmax was (425nm).
Curcumin showed three peaks with average area (X1=87279, X2=881260, X3=7641952),
at average retention time (T1=6.31, T2=6.87, T3=7.50) min with relative standard deviation (RSD%) (0.83, 0.08, 0.01) respectively, the sum of the average areas of the peaks was (Xt=8610491).
The calibration curve for curcumin and its three compounds was characterized by good
linearity within a range of concentrations ranging from (0.001-40)ppm. The recoverability R% was calculated and was (99.66%). The limit of quantitative detection LOQ was (0.056 ppb), and the qualitative limit of detection LOD was (0.009 ppb). The results confirm the possibility of adopting this method to determine curcumin and its various compounds.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The authors retain the copyright and grant the right to publish in the magazine for the first time with the transfer of the commercial right to the Tishreen University Journal -Basic Sciences Series
Under a CC BY- NC-SA 04 license that allows others to share the work with of the work's authorship and initial publication in this journal. Authors can use a copy of their articles in their scientific activity, and on their scientific websites, provided that the place of publication is indicted in Tishreen University Journal -Basic Sciences Series . The Readers have the right to send, print and subscribe to the initial version of the article, and the title of Tishreen University Journal -Basic Sciences Series Publisher
journal uses a CC BY-NC-SA license which mean
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
- The licensor cannot revoke these freedoms as long as you follow the license terms.
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.