The identification and detection of curcumin and its constituent compounds using high-performance liquid chromatography (HPLC) technology, and determination of the optimal conditions for the developed analytical method

Authors

  • Riham Moalla Tishreen University
  • Muhammad Maarouf Tishreen University
  • Tareq Arraj Tishreen University
  • Wissam Zam Tartous university

Abstract

This study aimed to develop a sensitive and accurate analytical method to determine and

detect curcumin using high-performance liquid chromatography HPLC. Optimal conditions such as mobile phase, temperature, flow speed, and the maximum wavelength for analysis λmax were studied. The study was carried out on a standard solution of curcumin with a concentration of (40ppm) and a separation column (C18) was used with specifications (250 ×4.6m.m.5μm).

The results showed the optimal conditions were as follows: the flow speed of the mobile

phase was (0.8 ml/min) as a mixture of acetonitrile and phosphoric acid (0.1%) in a ratio of (40:60 v/v), the temperature of the separation column was (40 ̊C), while λmax was (425nm).

Curcumin showed three peaks with average area (X1=87279, X2=881260, X3=7641952),

at average retention time (T1=6.31, T2=6.87, T3=7.50) min with relative standard deviation (RSD%) (0.83, 0.08, 0.01) respectively, the sum of the average areas of the peaks was (Xt=8610491).

The calibration curve for curcumin and its three compounds was characterized by good

linearity within a range of concentrations ranging from (0.001-40)ppm. The recoverability R% was calculated and was (99.66%). The limit of quantitative detection LOQ was (0.056 ppb), and the qualitative limit of detection LOD was (0.009 ppb). The results confirm the possibility of adopting this method to determine curcumin and its various compounds.

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Published

2024-11-02

How to Cite

1.
معلا ر, محمد معروف, طارق عراج, وسام زم. The identification and detection of curcumin and its constituent compounds using high-performance liquid chromatography (HPLC) technology, and determination of the optimal conditions for the developed analytical method. TUJ-BA [Internet]. 2024Nov.2 [cited 2024Dec.4];46(3):113-32. Available from: https://journal.tishreen.edu.sy/index.php/bassnc/article/view/17469

Issue

Vol. 46 No. 3 (2024): Tishreen University Journal of Research and Scientific Studies - Basic Sciences Series

Section

Articles

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