دراسة تحليلية حول مقارنة الشروط المثلى لتحديد التتراسيكلين باستخدام عمودي الفصل الكروماتوغرافييين C8 و C18 وفق تقانة HPLC- DAD
Abstract
يهدف هذا البحث إلى تحديد الشروط المثلى لفصل مركب التتراسيكلين وتحديده باستخدام
عمودي الفصل الكرموتوغرافيينC8 و C18 والمقارنة بينهما بغية تطوير طريقة سريعة وحساسة، يُمكن
اعتمادها في قياس هذا المركب باستخدام تقانة كروماتوغرافيا السائلة عالية الأداء وكاشف المصفوفة الديودية
(High Performance Liquid Chromatography – Diode Array Detector HPLC-DAD).
اعتمدت هذه الدراسة على تغيير أحد العوامل (نسب الطور المتحرك، درجة حرارة، سرعة تدفق الطور المتحرك، وطول موجة الامتصاص الأعظمي) وتثبيت العوامل الأخرى بغية الوصول إلى أفضل فصل مُمكن مترافقاً مع أفضل زمن احتفاظ ومساحة قمة للمركب المدروس. أظهرت الدراسة أفضلية عمود الفصل الكروماتوغرافي C8 مقابل عمود C18 لجهة مساحة القمة المفصولة 6.080 وزمن الاحتفاظ 4.169 ونقاوة الطيف 1000 للتركيز نفسه لمركب التتراسيكلين. تميز منحني المُعايَرة بخطية جيدة ومُعامل ارتباط R2= 0.99971 ممتاز ضمن مجال من التراكيز تراوح بين (1-100ppb).
The aim of this research is to determine the optimal conditions for separation and determination of the Tetracycline compound using both chromatographic columns C8 & C18. The comparison between the above mention columns was made in order to develop a sensitive and rapid method which can be used to measuring this compound by High Performance Liquid Chromatography – Diode Array Detector HPLC-DAD.
This study depends on changing one of the factors (Mobile phase ratio , Temperature, Flow- rate of the mobile phase, wavelength of the maximum absorption) and fixing the other factors in order to achieve the best possible separation conjugated with the best retention time (Rt) and the best peak area for the studied compound. The study showed that the area of the separated peak (6.080), the retention time (4.169) and the purity 1000 of the spectrum for the same concentration of Tetracycline were preferable by using chromatographic column C8. This result gives a preference for the chromatographic column C8 comparison to column C18. The calibration curve is found to have a good linearity and an excellent correlation coefficient R2= 0.99971 within a range of concentrations between(1-100ppb).
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The authors retain the copyright and grant the right to publish in the magazine for the first time with the transfer of the commercial right to the Tishreen University Journal -Basic Sciences Series
Under a CC BY- NC-SA 04 license that allows others to share the work with of the work's authorship and initial publication in this journal. Authors can use a copy of their articles in their scientific activity, and on their scientific websites, provided that the place of publication is indicted in Tishreen University Journal -Basic Sciences Series . The Readers have the right to send, print and subscribe to the initial version of the article, and the title of Tishreen University Journal -Basic Sciences Series Publisher
journal uses a CC BY-NC-SA license which mean
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
- The licensor cannot revoke these freedoms as long as you follow the license terms.
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.