In- house preparation of modified Kleihauer-Betke test reagents for estimation of fetomaternal hemorrhage

Authors

  • Mariam Mouna Tishreen University
  • Suzanne Alshemali Tishreen University
  • Maram Bilal Tishreen University

Abstract

Fetomaternal hemorrhage (FMH) refers to the entry of fetal blood into maternal circulation before or during delivery. In addition to its role in diagnosing idiopathic neonatal anemia, estimation of FMH is essential in  the passive prophylaxis  programs of hemolytic disease of the fetus and newborn (HDFN) by the appropriate administration of RhDIg to RhD negative women following delivery of D positive babies, or after exposure to a sensitizing event during pregnancy. The Kleihauer-Betke test (KBT) is still considered one of the most important tests in the detection of fetal red blood cells within the maternal circulation. KBT principle is based on the   the characteristic resistance of fetal hemoglobin (HbF) to acid elution, in contrast to adult hemoglobin (HbA). In this study, we aimed to prepare in –house reagents needed in the KBT  to use them in the detection of fetal hemoglobin and the estimation of FMH ,thus alleviating the need to import the highly expensive kits .

The modified Kleihauer-Betke method according to Clayton 1963 was adopted in our study, and the required reagents were prepared according to the method depicted in the American Association of Blood Banks (AABB) Technical Manual. Negative controls and different dilutions of positive controls were prepared to test, i the efficinecy of our locally prepared reagents,. Our experiments were carried out at the Central Haematology Laboratory in Tishreen University Hospital. PH measurements of the regeants were performed at a regular intervals throughout a storage period of 60 days, along with their effieveincy in HbF detection and estimation), and the, and both were shown to be constant. KBT using local reagents showed good accuracy in detecting fetal cells with a little tendency to overestimate FMH. According to our results, we can consider this study as a starting point for the use of this reagent in the investigation of unexplained neonatal anemia, and in conducting larger studies of FMH determination in women, thus helping to improve and standardize RhDIg administration.

Downloads

Published

2024-06-06

How to Cite

1.
منى م, سوزان الشمالي, مرام بلال. In- house preparation of modified Kleihauer-Betke test reagents for estimation of fetomaternal hemorrhage. Tuj-hlth [Internet]. 2024Jun.6 [cited 2024Jun.30];46(2):311-20. Available from: https://journal.tishreen.edu.sy/index.php/hlthscnc/article/view/17092

Issue

Vol. 46 No. 2 (2024): Tishreen University Journal for Research and Scientific Studies - Health Sciences Series

Section

Master student researches

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

The authors retain the copyright and grant the right to publish in the magazine for the first time with the transfer of the commercial right to Tishreen University Journal for Research and Scientific Studies - Health Sciences Series

 Under a CC BY- NC-SA 04 license that allows others to share the work with of the work's authorship and initial publication in this journal. Authors can use a copy of their articles in their scientific activity, and on their scientific websites, provided that the place of publication is indicted in  Tishreen University Journal for Research and Scientific Studies - Health Sciences Series   .  The Readers have the right to send, print and subscribe to the initial version of the article, and the title of  Tishreen University Journal for Research and Scientific Studies - Health Sciences Series  Publisher

journal uses a CC BY-NC-SA license which mean

You are free to:

  • Adapt — remix, transform, and build upon the material
  •  
  • The licensor cannot revoke these freedoms as long as you follow the license terms.

 

  • Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • NonCommercial — You may not use the material for commercial purposes.
  • ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
  • No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.