دراسة نسيجية وكيميائية نسيجية مناعية للبلعميات الكبيرة في الغدد اللعابية الشاهدة والمعالجة بالأيزوبروترينول في ذكر الفأر الأبيض البالغ
Abstract
Salivary glands are important effecter site in the mucosal immune network that possesses monocytic population which regulates and mediates the immune response contributing to the protection of oral surface. Isoprenaline is a sympathomimetic beta adrenergic. It is structurally similar to epinephrine but acts selectively on beta receptors. This work was carried out in order to study the macrophages in the major salivary glands in control and isoproterenol treated adult male albino rat. Salivary glands specimens were processed for histological study by LM & EM and stained immunohistochemically for detection of macrophage using CD68. LM examination of the major salivary gland sections of isoproterenol treated group revealed wide monocytic infiltration in their connective tissue septa. Immunohistochemical study displayed significant increase in the CD68 positive cells in the submandibular and specifically and more frequently in the sublingual salivary glands. Several forms of macrophages in different functional states were identified in the form of resident, adherent migrating, activated and giant macrophages. EM examination revealed many activated macrophages. It could be concluded that population composition of salivary macrophage is different in salivary glands, and its role in mucosal oral immunity.
تلعب الغدد اللعابية دوراَ كبيراَ فى المناعة الموضعية للفم وذلك لاحتوائها على نسبة كبيرة من الخلايا أحادية النوى. يعد الأيزوبرينالين (أيزوبروترينول) أحد الأدوية التى تماثل الأدرينالين فى تركيبه وعمله. لقد أجرى هذا البحث لدراسة بلعميات الغدد اللعابية الضابطة والمعالجة بلأيزوبروترينول فى ذكر الفأر الأبيض البالغ. تم أخذ عينات الغدد اللعابية لفحصها بالمجهر الضوئى والإلكتروني والكيمياء النسيجية المناعية باستخدام CD68 للكشف عن هذه البلعميات. أظهر المجهر الضوئى للمجموعة المحقونة بلأيزوبروترينول وجود ارتشاح خلايا وحيدات النوى كثيرة فى النسيج الضام, كما لوحظ زيادة ذات دلالة إحصائية فىالتفاعل المناعى للبلعمياتCD68 فى الغدد التحت فكية وخصوصاً في الغدة تحت لسانية. كما لوحظ وجود خلايا وحيدة النواة ملاصقة للغشاء البطانى للأوعية الدموية وخلايا مهاجرة ونشطة وعملاقة. كما أظهر المجهر الإلكتروني وجود العديد من البلعميات النشطة.
نستنتج من هذه الدراسة اختلاف في توزع مجاميع البلعميات فى الغدد اللعابية. وهذا يعطى مؤشراَ هاماَ على دور هذه الغدد فى المناعة الموضعية لمخاطية الفم.
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