مؤشر انقسام المنسليات المنوية في خصى الفئران المعالجة بجرعات متزايدة من الميتوتركسات
Abstract
حُقنت الفئران الذكور من سلالة Swiss عضليّاً بجرعات متزايدة من عقار الميتوتركسات Methotrexate MTX)) (25، 50، 100، 150، 200 ملغ/كغ) لمرّة واحدة فقط، ثم دُرست بعد مرور 15 يوماً.
حُسبت متوسّطات مجموع المنسليات المنوية Spermatogonia في المقطع العرضي للأنبوب المنوي، كما حسبت متوسّطات مجموع المنسليات المنوية التي دخلت في الطور التالي من الانقسام الخيطي المتساوي، وحسب أيضاً المؤشّر الانقسامي Mitotic Index (MI) للمنسليات المنوية, و تمّ تحليل النتائج إحصائياً باستخدام برنامج IBM-Spss 19، و قورنت المتوسّطات باستخدام اختبار : one-way ANOVA: Post Hos Test, Multiple comparisons, Tukey HSD ، وحلّل المؤشّر الانقسامي (MI) باستخدام اختبار Target Variable.
أظهرت النتائج أن حقن الـMTX لم يؤدِّ إلى انخفاض ذي دلالة إحصائية في متوسّطات مجموع المنسليات المنوية للمجموعات التجريبية مقارنة بالشاهد، بينما أدّى إلى انخفاض تدريجي متناسب مع ازدياد الجرعة في متوسّط مجموع المنسليات المنوية التي دخلت في الطور التالي من الانقسام الخيطي، حيث وصل هذا المتوسط إلى قيمة متدنّية جدّاً في الجرعتين: 150، 200 ملغ/كغ (2.267.10 و 2.153.65 على التوالي) مقارنة بالشاهد (9.437.40 )، ممّا أدّى إلى انخفاض شديد في المؤشّر الانقسامي (20.53% و 6.36% في الجرعتين 100 و200 ملغ/كغ على التوالي) مقارنة بالشاهد (61.79%).
Increasing dose of Mithotrexate (25, 50, 100, 150, 200 mg/kg) were tested on Swiss strain mouse by one intramuscular injection, and their effects were studied (15) days later.
The mean values of total spermatogonia and those of them undergoing mitotic division (in the metaphase) were evaluated in seminiferous tubular cross section, and the mitotic index (MI) of the spermatogonia was also calculated.
Statistical analysis was performed by using IBM-Spss 19 program and for mean values comparison (one way ANOVA: post Hos Test, Multiple Comparison, and Tukey HSD) was used, while mitotic index was calculated by using Target Variable.
Our results showed that Methotrexate injection didn’t lead to a significant decrease in spermatogonia mean values in the tested animals compared to the control, while it led to a dose dependent gradual decrease in the mean values of the spermatogonia undergoing mitotic division , and the greatest reduction was noticed in the two high doses: 150, 200, mg/kg (7.10±9.4, 3.65±2.15 respectively) compared to the control (37.40±2.26), and which led also to a severe reduction in the mitotic index (20.53%, 6.36% in the doses: 100, 200 mg/kg respectively) compared to the control (61.79%).
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The authors retain the copyright and grant the right to publish in the magazine for the first time with the transfer of the commercial right to the Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series
Under a CC BY- NC-SA 04 license that allows others to share the work with of the work's authorship and initial publication in this journal. Authors can use a copy of their articles in their scientific activity, and on their scientific websites, provided that the place of publication is indicted in Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series . The Readers have the right to send, print and subscribe to the initial version of the article, and the title of Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series Publisher
journal uses a CC BY-NC-SA license which mean
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
- The licensor cannot revoke these freedoms as long as you follow the license terms.
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
