Isolation of local samples of Bacillus thuringiensis and testing its effectiveness in the laboratory against the red two-spotted spider Tetranychus urticae Koch.

Authors

  • Lama hasan Tishreen University
  • Omiema Nasser Tishreen University
  • Majida mefleh General Authority for Agricultural Scientific Research

Abstract

Isolation of Bacillus thuringiensis from the local soil in Lattakia Governorate, the bacterial isolates were characterized by some characteristics based on their Gram staining, some biochemical properties and depending on the color characteristics on specific and selective cultures.

The isolates were tested against the Koch red two-spotted spider. Tetranychus urticae, under laboratory conditions, on stages (egg, nymph, and adult).

The results of the tests showed that the isolates had a significant effect on the egg stage, as the concentration of 107 cells / ml gave the highest mortality rate of 82%, with a significant difference from the rest of the treatments.. Mortality in other stages was (43-92) % for larvae, (47-95) % for nymphs of the first age, (56-90) % for nymphs of the second age, and (44-91) % for adult females.

The control mortality rates ranged between (0-5) % for larvae, (1-8) % for first-instar nymphs, (3-11) % for second-instar nymphs, (4-7) % and (4-7) % for adult females.

 

Published

2024-01-26

How to Cite

حسن ل., أميمة ناصر, & ماجدة مفلح. (2024). Isolation of local samples of Bacillus thuringiensis and testing its effectiveness in the laboratory against the red two-spotted spider Tetranychus urticae Koch. Tishreen University Journal -Biological Sciences Series, 45(6), 45–57. Retrieved from https://journal.tishreen.edu.sy/index.php/bioscnc/article/view/15815