دراسة تأثير بعض منظمات النمو في الإكثار الدقيق لنبات العرن المثقب Hypericum perforatum

Authors

  • حسين العذبة

Abstract

detailed in vitro multiplication system for rapid micropropagataion of the Hypericum perforatum L, has been developed by using  tissue culture technique . The  shoots (1- 0.5cm) were cultured on  MS ( Murashige and Skoog 1962) then placed onto  based medium containing a combination of growth regulators at different  concentrations (BA , Kin , IBA ) . After that they were placed into MS based medium containing NAA and IBA.   Results showed that the highest number of growth was 39.6 when ( 0.6 mg/l BA + 0.1 mg/l IBA)  were adding (MS3), also the highest of the growth was (3,81cm) in medium MS3 .The observed rooting was (7.1roots , 10,5 cm ) length and %95 in percentage of rooting) in R5 (MS+1mg/l NAA) , and acclimatization was 85%

 

تم تطوير تقنية لإكثار نبات  Hypericum  perforatum في المختبر بإستعمال تقنية  زراعة النسج النباتية من خلال عقل بطول (1- 0.5) سم والتي زرعت على وسط (Murashige and Skoog ,1962)    ( MS)وبعد ذلك تم إكثارها على وسط MS المضاف إليه منظمات نمو مختلفة ( البنزيل أدنينBA ،الكينتين Kin وإندول بيوتريك أسيد IBA ( حمض الزبدة )  ) . أوضحت النتائج أن أكبر نسبة لمتوسط  النموات المتشكلة كانت 39.6 في المعاملة MS3 التي تحتوي IBA ( 0.1 mg/l )  + BA     ( 0.6 mg/l )  وكان  أكبر  طول للنموات أيضاٌ على المعاملة   MS3حيث بلغ أكبر طول للنموات 3.81  سم . تمت بعد ذلك عملية التجذير على أوساط  MS  المضاف إليها IBA أو NAA ) نافتيل حمض الخل (و كانت المعاملة R5 التي تحتوي على NAA  (mg/L  1) هي الفضلى حيث بلغت  نسبة التجذير %95  و متوسط عدد الجذور  7.1 سم , ومتوسط طولها 10.5 سم . بلغت نسبة الأقلمة %85 من عدد النموات التي أمكن الحصول عليها.

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Published

2018-11-01

How to Cite

العذبة ح. (2018). دراسة تأثير بعض منظمات النمو في الإكثار الدقيق لنبات العرن المثقب Hypericum perforatum. Tishreen University Journal -Biological Sciences Series, 40(3). Retrieved from https://journal.tishreen.edu.sy/index.php/bioscnc/article/view/4427

Issue

Vol. 40 No. 3 (2018): العلوم البيولوجية

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