A study of Some Factors Affecting the Vegetative Micropropagation of Ornamental Viburnum Bush (Viburnum Tinus L.)
Abstract
This study was conducted during 2012-2013 in order to efficiently micropropagate viburnum (Viburnum tinus) bushes using tissue culture techniques. The shoots were cultured on a Murashige and Skoog medium supplemented with 30 gl-1 sucrose, 7 gl-1 agar - agar, citric acid as an anti-oxidant (300 mgl-1), and different concentrations of benzyl amino purine and Naphthalene Acetic Acid. A media of Murashige and Skoog was used for laboratory rooting after reducing the major mineral elements to the half, reducing the sucrose to 20 gl-1, and addingindole-3-butyric acid (IBA) of different concentrations (0, 0.5, 1, 1.5 mgl-1). The results showed that it is necessary to have auxin and cytokinin in culture to improve the value of open buds and the number of shoots per initial explants. The concentrations 0.25 mgl-1 from NAA with 1 mgl-1 from BAP gave the highest value of open buds (93.33%) and the maximum number of shoot per initial explants (1.57). To improve the number and length of the shoots produced, the solution mineral (MS) was replaced with another: media woody plants (WPM) which gave better elongation for the resulting growths (3.21 cm) and a better number of shoots (2.72 Growth/explant) compared to the media (MS) using the same compatibility hormone (0.25 mgl-1 of NAA and 1 mgl-1 of BAP).
The results also show that the highest percentage of rooting reached (84.44%) with (0.5 mgl-1) IBA which was better than (1.5 mgl-1) IBA and better than the treatment of the control. Results also showed that the best medium for the length and number of roots formed was (2.7cm, 3.82root) when the concentration was (0.5 mgl-1) surpassing the control. The success rate of the acclimatization of the resulting laboratory plantlets under glasshouse conditions reached (73.33%) one month and a half after the transplanting.
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