Comparison between gel and tube methods in antibody screening by using Indirect Coombs in hemoglobinopathies patients in Latakia

Authors

  • Suzanne Alshimali Tishreen University
  • Mohamad Ayman Awama Tishreen University
  • Saraa Baddour Tishreen University

Abstract

Antibody screening is of great importance both before blood transfusion, and in the diagnosis of hemolytic transfusion reaction (HTR) and hemolytic disease of the fetus and newborn (HDFN). Anti-human Globulin Test (AHGT), also known as Coombs Test, remains the most important test that is widely used in antibodies detection. The standard gold method for conducting this test has been the conventional tube test, but this method is not without some disadvantages, so other better methods were developed and came into use such as, solid phase tests; which include the Gel Test. This technique has spread rapidly because of its speed, ease of performance, high sensitivity and specificity as reported by several studies, although some other reports described a relative increase in some nonspecific reactions.

In this study, we aim to compare the tube and gel methods in detecting antibodies formed against red blood cells alloantigens in thalassemia and sickle cell disease patients in Latakia.

We conducted antibodies screening by gel and tube methods in parallel for 70 patients using IAT in Liss-coombs at 37º, and compared the results of both methods.

According to our findings, the tube method remains a good option with high sensitivity and specificity.

Author Biographies

Suzanne Alshimali, Tishreen University

Assistant  Professor, Department of Laboratory Medicine, Faculty of Medicine

Mohamad Ayman Awama, Tishreen University

Assistant  Professor, Department of Biochemistry and Microbiology, Faculty of Pharmacy

Saraa Baddour, Tishreen University

Postgraduate Student, Department of Biochemistry and Microbiology, Faculty of Pharmacy

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Published

2022-03-29

How to Cite

1.
الشّمالي س, عوّامة مأ, بدّور س. Comparison between gel and tube methods in antibody screening by using Indirect Coombs in hemoglobinopathies patients in Latakia. Tuj-hlth [Internet]. 2022Mar.29 [cited 2024Nov.24];44(1):209-20. Available from: https://journal.tishreen.edu.sy/index.php/hlthscnc/article/view/12016

Issue

Vol. 44 No. 1 (2022): Tishreen University Journal for Research and Scientific Studies - Health Sciences Series

Section

Master student researches

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