استخدام تقنية PCR-RAPD في دراسة التباينات الوراثية لمجموعة من الطرز الوراثية من الجرجير .officinale Nasturtium المنتشرة في المنطقة الساحلية.
Abstract
يشير هذا البحث إلى إمكانية استخدام تقنية التفاعل التسلسلي للبوليمراز / PCR- RAPD / بهدف معرفة التباينات الوراثية من خلال تحديد الهوية الوراثية للطرز المدروسة، حيث استخدم في هذا البحث عدد من البوادئ Primers ( 8 بوادئ )، وتم حساب معامل التشابه والبعد الوراثي باعتماد طريقة ( Nei and Li., 1979 ) ، فأظهرت نتائج الرحلان الكهربائي Electrophoresis اختلافاً في عملية مكاثرة الـ DNA Amplification تبعاً للبادئ المستخدم، وتبين أن هناك بوادئ لم يكن لها مقاطع متممة في DNA الطرز المدروسة، بينما كان هناك مكاثرة للـ DNA عند القسم الأخر من البوادئ.
وتبين لنا من هذه الدراسة ومن حساب معامل التشابه والبعد الوراثي للطرز المدروسة ومقارنة النتائج مع بعضها البعض أن معامل التشابه بين الطرز المختلفة يتراوح ما بين ( 0,996 – 0,369 ) وبالتالي فإن أكبر بعد وراثي كانت قيمته / 0,631 /، وأصغر بعد وراثي كانت قيمته 0,004. وإن هناك تدرج في التباينات الوراثية للطرز المدروسة ما بين هذه القيم المحسوبة لمعامل التشابه والبعد الوراثي.
This study indicates the possibility of using PCR -RAPD technique for studying the genetic diversity by identifying the genetic identity of studied genotypes.
Eight operon primers have been used in this study. The genetic similarity and distance coefficients were calculated using (Nei and Li., 1979) procedure. The results of electrophoresis showed variations in DNA amplification according to primers used.
Some primers gave no complimentary fragments to the DNA of studied genotypes whereas other primers gave help for DNA amplifications. The genetic similarity coefficient among studied genotypes was between 0,369 and 0,996, and the largest and smallest genetic coefficients were 0,63 and 0,004 respectively, with gradual values of genetic diversity among studied genotypes for genetic similarity and distance coefficients.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The authors retain the copyright and grant the right to publish in the magazine for the first time with the transfer of the commercial right to the Tishreen University Journal -Basic Sciences Series
Under a CC BY- NC-SA 04 license that allows others to share the work with of the work's authorship and initial publication in this journal. Authors can use a copy of their articles in their scientific activity, and on their scientific websites, provided that the place of publication is indicted in Tishreen University Journal -Basic Sciences Series . The Readers have the right to send, print and subscribe to the initial version of the article, and the title of Tishreen University Journal -Basic Sciences Series Publisher
journal uses a CC BY-NC-SA license which mean
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
- The licensor cannot revoke these freedoms as long as you follow the license terms.
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.