Virulence of Syrian Entomopathogenic Nematode Isolates Against of Galleria mellonella (Lepidoptera: Pyralidae)

Authors

  • Mohamad Ahmad Tishreen University
  • Nada Allouf Tishreen University
  • Mai Ali Tishreen University

Abstract

The virulence of three native isolates of Entomopathogenic Nematodes (EPNs) Heterorhabditis 160, Heterorhabditis 136, Steinernema 22, was evaluated using the last instar of Galleria mellonella as host. Four bioassays were conducted to compare them. (i) Exposure time assay, the isolate H.136 recorded the highest mortality rate within 15 minutes. Less significant differences in mortality rates among isolates were observed by increasing the exposure time intervals. (ii) Dose-response assay, all the studied isolates showed 100% mortality rate at the dose of 40 infective juveniles (IJ) per insect larva. H.136 caused highest mortality rate (41.66%), at the dose of 5 IJ/L. Where the isolate S. 22 revealed the lowest mortality rate (20.33%). (iii) One-On-One assay, the mortality rates among all isolates presented close results, where the isolate H.136 was the highest. (iv) Sand column assay, the results showed that the ability of isolates to find, penetrate and kill the host, with significant difference between them. Propit analysis was conducted to calculate the lethal half-time (LT50) and lethal half-dose (LD50). The findings presented that the most virulence isolate H.136 leaded 22 minutes as LT50 and 5.42 infective juveniles per larva as LD50.

Author Biographies

Mohamad Ahmad, Tishreen University

Professor, Department of plant Protection, Faculty of Agriculture

Nada Allouf, Tishreen University

Assistant Professor, Department of plant Protection, Faculty of Agriculture

Mai Ali, Tishreen University

PhD student, Department of plant Protection, Faculty of Agriculture

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Published

2022-06-06

How to Cite

أحمد م. ., ألوف ن. ., & علي م. . . (2022). Virulence of Syrian Entomopathogenic Nematode Isolates Against of Galleria mellonella (Lepidoptera: Pyralidae). Tishreen University Journal -Biological Sciences Series, 44(2), 71–80. Retrieved from https://journal.tishreen.edu.sy/index.php/bioscnc/article/view/12402

Issue

Vol. 44 No. 2 (2022): Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series

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