تغذية الأفراد البالغة ويرقات محار اللؤلؤ Pinctada radiata (Leach, 1814)باستخدام الغذاء الطّحلبي الحيّ في المختبر
Abstract
This research dealt, with the production of live food composed of green monocyte alga Tetraselmis sp. which is suitable for growth of adult individuals and larvae of pearl oyster Pinctada radiata, and the determination of the appropriate food concentrations for adults and larvae during breeding and hatching in vitro.
The required alga was isolated from marine water in the Afamia Region of the Lattakia Coast, and culture using the medium of solid agar, in addition to the media of nutrient stock F/2 (15 – 30) day before the transfer of adults to the breeding tanks in the laboratory.
The average number of algal cells of Tetraselmis sp. ranged between 9.17X104 on the first day of the brood and 100X104 on the day 15, while the highest value was on the day 10 with an average 144.17X104.
The daily ration from the alga cells for one adult was0.1176 g equivalent to 408 ml from the algal suspension, while the daily feeding volume of the larvae ranged between 21 – 33 ml for 6 L tank.
تناول هذا البحث إنتاج الغذاء الحيّ المؤلف من الطّحلب الأخضر وحيد الخلية Tetraselmis sp. المناسب لنموّ أفراد ويرقات محار اللؤلؤ Pinctada radiata، وتحديد التراكيز المناسبة الواجب إضافتها لكل من الأفراد البالغة واليرقات أثناء التربية والتفريح مخبرياً.
تمّ عزل الطّحلب المطلوب للتغذية من المياه البحرية في منطقة أفاميا من ساحل مدينة اللاذقية، وتنّميته باستخدام وسط الآغار الصلب، بالإضافة إلى مخزون الأملاح المغذّية F/2، قبل (15 – 30) يوم من نقل الأفراد البالغة إلى أحواض التربية في المختبر.
تراوح متوسط عدد الخلايا الطحلبية لـ Tetraselmis sp. بين 9.17X104 في اليوم الأول من الحضن و 100X104 في اليوم الخامس عشر، بينما كانت القيمة العليا في اليوم العاشر إذ بلغ المتوسط .144.17X104
بلغت الوجبة اليومية للفرد البالغ الواحد من الخلايا الطحلبية 0.1176 غ أي ما يعادل 408 مل من المعلّق الطّحلبي، بينما تراوح حجم التغذية اليومي لليرقات بين 21 – 33 مل لحوض سعة 6 ل.
References
ALAGARSWAMI, K.; DHARMARAJ, S.; VELAYUDHAN, T. S.; CHELLAM, A.; VICTOR, A.C.C. and GANDHI, A. D. Larval rearing and production of spat of pearl oyster Pinctada fucata (Gould). Aquaculture, No. 34, 1983, 287—301.
CHELLAM, A. Biology of pearl oyster. C.M.F.R.I. Bull., No. 39, 1987, 13-20.
FAO. Training Manual on Pearl Oyster Farming and Pearl Culture. FAO Fisheries Circular, Central Marine Fisheries Research Institute at Tuticorin, India, Regional Sea farming Development and Demonstration Project (RAS/90/002), 1991, 64.
FAO. Manual on the production and use of live food for aquaculture. FAO Fisheries Technical Paper, No. 361, Rome, 1996, 295.
FAO. Review of the state of world aquaculture. FAO Fisheries Circular, No. 886, Rev. 2, Rome, 2003, 95.
FAO. Hatchery culture of bivalves. A practical manual. FAO Fisheries Department, Rome, 2007, 175.
FAO. Aquaculture development. 4. Ecosystem approach to aquaculture. No. 5, Suppl. 4, Food and Agriculture Organization of the United Nations, Rome, 2010, 65.
GALIL, B.S. and ZENETOS. A. A sea change: Exotics in the eastern Mediterranean Sea. In: Leppäkoski E, Gollasch S, Olenin S, editors. Invasive Aquatic Species of Europe. Distribution, Impacts and Management. Netherlands: Kluwer Academic Publishers, 2002, 325–336.
GERVIS, M.H.; SIMS, N.A. The Biology and Culture of Pearl Oysters (Bivalvia: Pteriidae). ICLARM Stud , Rev., No. 21, 1992, 49.
GOFAS, S. and ZENETOS, A. Exotic mollusks in the Mediterranean basin, current status and perspectives. Oceanography and Marine Biology, Vol. 41, 2003, 237–277.
GRIFFITH, G.W.; MURPHY KENSLOW, M.A. and ROSE, L.A. A mass culture method for Tetraselmis sp. – a promising food for larval crustaceans. In: J. W. Avault. Jr. (ed) Proceedings of the 4th Annual Workshop of the World Mariculture Society. Louisiana State University, Baton Rouge, 1973, 289–294.
GUILLARD, R.L. Culture of phytoplankton for feeding marine invertebrates. In: P.B. Smith (ed) Culture of Marine Invertebrates. Plenum Press, New York, 1975, 29–60.
LAING, I. and HELM, M.M. Factors affecting the semi-continuous production of Tetraselmis suecica (Kylin) Butch. in 200 l vessels. Aquaculture, No. 22, 1981, 137–148.
LAING, I. and JONES, E. Large scale turbidostat culture of marine microalgae. Aquacultural Engineering, No. 2, 1983, 203–212.
LAING, I. and MILLICAN, P. F. Relative growth and growth efficiency of Ostrea edulis L. spat fed various algal diets. Aquaculture, No. 54, 1986, 245–262.
LAING, I. and AYALA, F. Commercial mass culture techniques for producing microalgae. 1987, 447–477. In: Akatsuka (ed) Introduction to Applied Phycology. Academic Publishing, The Hague, The Netherlands.
LAING, I. Nutritional value of dried algae diets for larvae of Manila clam, Tapes philippinarum. J. Mar. Biol. Assoc., UK, No. 70, 1990, 1–12.
LAING, I. and PSIMOPOULOUS, A. Hatchery culture of king scallop (Pecten maximus) spat with cultured and bloomed algal diets. Aquaculture, No. 169, 1998, 55–68.
LE MOULLACA, G.; SOYEZA, G.; SHAM-KOUAA, M.; LEVYA, P.; MORICEAUA, J.; VONAUA, V.; MAIHOTAA, M. and COCHARDB, J. Feeding the pearl oyster Pinctada margaritifera during reproductive Conditioning. Aquaculture Research, Vol. 44, No. 3, 2013, 404–411.
MUTHIAH, P.; RODRIGO, J.X.; SUJA, N. Larval rearing and spat production of Marcia opima (Gmelin). Aquaculture, 211, 2002, 393–401.
NAYAR NAGAPPAN, K.; RAJAPANDIAN, M.E.; GANDHI, A,D. and GOPINATHAN, C.P. Larval rearing and production of spat of the oyster, Crassostrea madrasensis (Preston) in an experimental hatchery. Indian J. Fish., No. 31, 1984, 233-243.
O’FOIGHIL, D. ; KINGZETT, B. ; O’FOIGHIL, G. O. and BOURNE, N. Growth and survival of juveniles scallops, Pateinopecten yessoensis, in nursery culture. J. Shellfish Res. 9 (1), 1990, 135–144.
ROSE, A.R. and BAKER, B.S. Larval and spat culture of the Western Australian silver- or gold lip pearl oyster, Pinctada maxima Jameson (Mollusca: Pteriidae). Elsevier Science B.V, Vol. 126, Issues 1–2, 1994, 35-50 .
STEIN, J. R. Handbook of Phycological Methods: Culture Methods and Growth Measurements. Cambridge University Press, Cambridge, England, 1973, 448.
TROTTA, P. A simple and inexpensive system for continuous monoxenic mass culture of marine microalgae. Aquaculture, No. 22, 1981, 383–387.
UTTING, S. D. and SPENCER, B. E. The hatchery culture of bivalve mollusc larvae and juveniles. Lab. Leafl., MAFF Fish. Res., Lowestoft, No. 68, 1991, 31.
WEBB, K. L. and CHU, F. L. E. Phytoplankton as a source of food for bivalve larvae. In: (eds: Pruder, G.D., Langdon, C. & Conklin, D.) Proceedings of the 2nd International Conference on Aquaculture Nutrition: Biochemical and Physiological Approaches to Shellfish Nutrition, October 1981, Rehoboth Beach, Delaware. Louisiana State University Press, Baton Rouge, 1983, 272–291.
WONG, T.H.; LIM, T.G. and RAI, H.S. Induced spawning, larval development and juvenile growth of Anadara granosa (L) in the laboratory. In: Proceedings of the workshop on the biology of Anadara granosa in Malaysia, Jan. 22-23, Penang, Malaysia, 1986, 10.
Downloads
Published
How to Cite
Issue
Section
License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The authors retain the copyright and grant the right to publish in the magazine for the first time with the transfer of the commercial right to the Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series
Under a CC BY- NC-SA 04 license that allows others to share the work with of the work's authorship and initial publication in this journal. Authors can use a copy of their articles in their scientific activity, and on their scientific websites, provided that the place of publication is indicted in Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series . The Readers have the right to send, print and subscribe to the initial version of the article, and the title of Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series Publisher
journal uses a CC BY-NC-SA license which mean
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
- The licensor cannot revoke these freedoms as long as you follow the license terms.
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.