توصيف عزلات Bacillus thuringiensis المعزولة من التربة السورية واختبار فاعليتها ضد يرقات فراشة طحين البحر الأبيض المتوسط Ephestia kuehniella (Lepidoptera: Pyralidae)

Authors

  • محمد أحمد
  • عيسى كبيبو
  • مايسة مهيار

Abstract

جرى البحث على ترب بعض النظم الطبيعية والزراعية في سوريا للبكتريا الممرضة للحشرات  Bacillus thuringiensis. تبين من خلال فحص المستعمرات البكتيرية بالإعتماد على الشكل المورفولوجي للبلورات والاختبارات الكيميائية الحيوية أن %97 من العزلات تابعة لبكتيريا B. thuringiensis، مما يشير إلى أن بكتيريا B. thuringiensis متوافرة بغزارة في الترب السورية المدروسة. صنفت 219 عزلة من بكتيريا B. thuringiensis الى 8 تحت أنواع بمساعدة الاختبارات البيوكيميائية وهي: تحت نوع kurstaki B. thuringiensis subsp. وكان الأكثر شيوعا". وتحت الأنواع: biotype 9، thuringiensis، kenyae، sotto، dendrolimus،finitimus like- و.pakistani  وجد أن 192عزلة (88%) من أصل 219 عزلة سامة للعمر اليرقي الثالث لفراشة طحين البحر الأبيض المتوسط Ephestia kuehniella، أعطت 50 عزلة منها نسبة قتل أعلى من 50 %. كما أظهرت النتائج أن فاعلية العزلات التي تحتوي على بلورات ذات شكل معين bipyramidal أعلى من فاعلية العزلات المحتوية على بلورات كروية الشكل.

The occurrence of entomopathogenic bacterium Bacillus thuringiensis was investigated for some natural ecosystems in Syrian coastal plain soils. In the present study, B. thuringiensis was found in all tested ecosystems in Syria. Among the examined colonies based on crystal protein formation and biochemical tests, the proportion identified as B. thuringiensis was 97%, that indicate that B. thuringiensis was highly abundant in Syrian soils. Tow hundred and nineteen strains were found belonging to eight biochemical types with B. thuringiensis subsp. kurstaki being the most common. The other biochemical types were referable to biotype 9, thuringiensis, kenyae, sotto, dendrolimus, like- finitimus and pakistani. Of the 219 strains 192 (88%) were toxic to the third instar larvae of Ephestia kuehniella, where 50 isolates caused more than 50% mortality. The frequency of toxicity was greater in strains that produced bipyramidal parasporal crystals rather than strains produced spherical crystals.

Downloads

Published

2019-01-13

How to Cite

أحمد م., كبيبو ع., & مهيار م. (2019). توصيف عزلات Bacillus thuringiensis المعزولة من التربة السورية واختبار فاعليتها ضد يرقات فراشة طحين البحر الأبيض المتوسط Ephestia kuehniella (Lepidoptera: Pyralidae). Tishreen University Journal -Biological Sciences Series, 34(2). Retrieved from https://journal.tishreen.edu.sy/index.php/bioscnc/article/view/6247

Issue

Vol. 34 No. 2 (2012): العلوم البيولوجية

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

The authors retain the copyright and grant the right to publish in the magazine for the first time with the transfer of the commercial right to the Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series   

 Under a CC BY- NC-SA 04 license that allows others to share the work with of the work's authorship and initial publication in this journal. Authors can use a copy of their articles in their scientific activity, and on their scientific websites, provided that the place of publication is indicted in Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series  .  The Readers have the right to send, print and subscribe to the initial version of the article, and the title of Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series    Publisher

journal uses a CC BY-NC-SA license which mean

You are free to:

  • Adapt — remix, transform, and build upon the material
  •  
  • The licensor cannot revoke these freedoms as long as you follow the license terms.

 

  • Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • NonCommercial — You may not use the material for commercial purposes.
  • ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
  • No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Most read articles by the same author(s)

<< < 1 2 3 4 5