In vitro efficacy of Rosmarinus officinalis L. leaves ethanolic extract and essential oil against head lice
Abstract
Introduction: Head lice infestation is one of the most important public health proplems among children worldwide, its treatment is challenging because of the toxicity and the increased resistance to the existing treatments. Therefore, it’s important to find alternative non-toxic anti-lice agents to overcome this problem.
Objective: This study aims to test the efficacy of essential oil and ethanolic extract from Rosmarinus officinalis L. leaves and compare their activity with benzyl benzoate in vitro.
Materials and methods: The leaves of Rosmarinus officinalis L. were collected from Banias City, Syria. Essential oil was extracted from dried and powdered leaves by hydrodistillation using a modified Clevenger apparatus. Ethanolic extract was prepared from leaves powder using Soxhelt. A filter paper diffusion bioassay was carried out to determine the pediculicidal activity of ethanolic extract and essential oil from Rosmarinus officinalis L. leaves and comparing their activity with benzyl benzoate lotion, 4µl/cm2 was an appropriate starting dose for screening.
Results: In a filter paper contact bioassay with P. humanus capitis females at 4 µl/cm 2 , the results showed that Rosemary essential oil ethanolic solution 800 µl/ml with LT50= 0.17h was more toxic than Rosemary ethanolic extract 25 mg/ml with LT50= 1.99h, but both were less effective than benzyl benzoate lotion with LT50= 0.03h.
Conclusion: The results demonstrate Rosemary essential oil in our study was more toxic than Rosemary essential oil in other studies and Rosemary ethanolic extract has good mortality against head lice, both of them can use in preparation the pediculicdal commercial product .
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The authors retain the copyright and grant the right to publish in the magazine for the first time with the transfer of the commercial right to Tishreen University Journal for Research and Scientific Studies - Health Sciences Series
Under a CC BY- NC-SA 04 license that allows others to share the work with of the work's authorship and initial publication in this journal. Authors can use a copy of their articles in their scientific activity, and on their scientific websites, provided that the place of publication is indicted in Tishreen University Journal for Research and Scientific Studies - Health Sciences Series . The Readers have the right to send, print and subscribe to the initial version of the article, and the title of Tishreen University Journal for Research and Scientific Studies - Health Sciences Series Publisher
journal uses a CC BY-NC-SA license which mean
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
- The licensor cannot revoke these freedoms as long as you follow the license terms.
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
