تأثير الملوحة (Nacl) في إنبات البذور ونمو البادرات المزروعة في المختبر لصنفين من نبات فول الصويا [ Glycin max (L.) Merr. ]
Abstract
تهدف الدراسة إلى مقارنة تأثير خمسة تراكيز ملحية (Nacl) مختلفة (0، 50، 100، 150، 200 ميلي مول) في إنبات البذور ونمو البادرات لصنفين من نبات فول الصويا (sb-172، sb-44) .
تمّ إنبات البذور في أطباق بتري على أوراق ترشيح معقمة ومرطبة بـ10 ميلي ليتر من الوسط المستخدم؛ وذلك في درجة حرارة 25 م˚ وفي الظلام . أظهرت النتائج التي حصلنا عليها تناقص النسبة المئوية للإنبات , طول الجذر والسويقة مع زيادة تركيز Nacl في الوسط ، وكانت أفضل القيم من الصنف sb-44 .
تمّت ملاحظة فروق معنوية بين الشاهد والبذور المعالجة بعد 7 أيام من الحضانة . زرعت البذور النابتة على وسط Murashige and Skoog الأساسي (MS) والصلب الذي يحتوي التركيز نفسه من الملوحة . كان نمو الجذور أكثر تأثراً بالتركيز 200 ميلي مول Nacl مقارنة بنمو البادرات . نقلت هذه الأخيرة , بعد ذلك , إلى أصص تحوي تربة مغذية (تورب), ورويت بماء ملحي (التركيز نفسه الذي نمت عليه), وتمت أقلمتها في المختبر .
حصلنا على نباتات بحالة خضرية جيدة, استمر نموها إلى مرحلة النضج خلال 12-10 أسبوعاً.
The objective of this study is to compare the effect of five different Nacl concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two cultivars of soybean (sb-44,sb-172).
Seeds were germinated in petri dishes with sterile filter paper moistened with 10 ml of test solution at 25 c˚ in dark .
The obtained results showed that germination percentage , length of root and hypocotyl were decreased with the increase of the Nacl concentration in the medium. The best values was from cultivar sb-44 .
After seven days of incubation , significant differences were observed between control and treated seeds .
Germinated seeds were subcultured on basal Murashige and Skoog medium solid at the same Nacl concentration . The roots growth was mor affected by 200 mM Nacl compared to seedling growth .
Plantlets were transfered into pots with nutrient soil , irrigated with saline water and were adapted at laboratory . we obtained good plants grew to maturity in 10- 12 weeks .
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