Partial Purification of laccase enzyme from rosemary leaves (Rosmarinus officinals) and study of some its properties
Abstract
In this study a partial purification of laccase enzyme was carried out from rosemary. The purification steps included precipitation with acetone 75% as a first step, then purification using ion exchange chromatography on a DEAE-Sephadex column, followed by gel filtration chromatography using a Sephadex G100 column. Yield at the end of the purification steps was 54.14%, with a fold purification of 40.86 and a specific activity of 44.54 units/mg. The studied laccase showed a highest activity at pH 7, while its optimum temperature was 65 °C using 2-2`azino-bis (3ethl benzothiazottin 6 sulfonic acid) (ABTS) as a substrate. The results showed the thermal stability of the enzyme, where 55% of its activity remained after being incubated for an hour at a temperature of 75 °C, and laccase enzyme maintained more than 92% of its activity after being incubated for 24 hours at pH between (6-9). It should be noted that the above properties of the studied laccase enzyme give it great importance in various industrial applications.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The authors retain the copyright and grant the right to publish in the magazine for the first time with the transfer of the commercial right to the Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series
Under a CC BY- NC-SA 04 license that allows others to share the work with of the work's authorship and initial publication in this journal. Authors can use a copy of their articles in their scientific activity, and on their scientific websites, provided that the place of publication is indicted in Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series . The Readers have the right to send, print and subscribe to the initial version of the article, and the title of Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series Publisher
journal uses a CC BY-NC-SA license which mean
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
- The licensor cannot revoke these freedoms as long as you follow the license terms.
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.