Vitamin Riboflavin production by two locally isolated yeast isolates and the effect of temperature and pH on their productivity

Authors

  • Aleen Sarko Tishreen University
  • Mysa'a Yazajy Tishreen University
  • Nesreen Nakshow Tishreen University

Abstract

In this study, a group of yeasts were isolated from different natural sources, then they were purified and tested for their ability to produce the vitamin Riboflavin. The optimum temperature and pH to produce the largest amount of the vitamin were determined. 21 pure yeast isolates were obtained from different sources which they were able to produce riboflavin in a synthetic production medium.

The results showed that two isolates of these yeasts superior in producing the largest amount of vitamin. The strains were identified by using the Carbohydrate fermentation method in the API strips (test kit), it turned out that they were: Candida famata and Candida guilliermondii. The ability of these two isolates to produce riboflavin differed according to the different conditions of temperature and pH applied in the experiments.

It was found that conditions of 30 ° C and pH = 6 were optimal to give the largest amount of vitamin. The isolate C. guilliermondii was the best as it gave an amount of vitamin amounted to 172.24 g / 50 ml production medium under these conditions. Compared to the isolate C.famata, which produced an amount estimated at 57.84 g/50 ml production medium under the same conditions. whereas the amount of produced vitamin decreased to 53.84 µg and 0.24 µg for C.gulliermondii and C.famata, respectively, when the applied conditions were 30°C and pH=4.5. On the other hand, it was found that the conditions of temperature 35 ° C and pH = 7 were not suitable for producing vitamin by both isolates.

Downloads

Published

2023-05-21

How to Cite

اليــن سركو, ميساء يازجي, & نسرين نقشو. (2023). Vitamin Riboflavin production by two locally isolated yeast isolates and the effect of temperature and pH on their productivity. Tishreen University Journal -Biological Sciences Series, 45(2), 235–248. Retrieved from https://journal.tishreen.edu.sy/index.php/bioscnc/article/view/14328

Issue

Vol. 45 No. 2 (2023): Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

The authors retain the copyright and grant the right to publish in the magazine for the first time with the transfer of the commercial right to the Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series   

 Under a CC BY- NC-SA 04 license that allows others to share the work with of the work's authorship and initial publication in this journal. Authors can use a copy of their articles in their scientific activity, and on their scientific websites, provided that the place of publication is indicted in Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series  .  The Readers have the right to send, print and subscribe to the initial version of the article, and the title of Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series    Publisher

journal uses a CC BY-NC-SA license which mean

You are free to:

  • Adapt — remix, transform, and build upon the material
  •  
  • The licensor cannot revoke these freedoms as long as you follow the license terms.

 

  • Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • NonCommercial — You may not use the material for commercial purposes.
  • ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
  • No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.