مقارنة بين الظروف المثلى لإنتاج أنزيم السيلّولاز من العزلتين الفطريتين المحليتينT.harzianum و A. alternate

Authors

  • سناء سارة

Abstract

تمّ الكشف عن قابلية العزلتين الفطريتين المحليتين Trichoderma harzianum و Alternaria alternate، المعزولتين من المحيط الجذري لنبات القمح Triticum aestivum L.، لإنتاج أنزيم السيلّولاز في الأوساط الصلبة CMC-Agar. فلوحظ أنّ للفطرين قابلية لإنتاج الأنزيم، وتفوق الفطر T.harzianum في إنتاجه للسيلّولاز على الفطر A.alternate. ثمّ أجريّ اختبار كمي باستخدام وسط مندل السائل لتحديد الظروف المثلى من (فترة حضن، درجة حرارة وpH) لأفضل إنتاج للسيلّولاز من الفطرين. فكانت أفضل ظروف لإنتاجه من الفطر T.harzianum هي 7 أيام حضن (3.97 U/mL) ، درجة حرارة 26C (4.07 U/mL) وpH=5.5 (4.19U/mL)، بينما أفضل ظروف لإنتاج السيلّولاز من الفطرA.alternate هي 8 أيام حضن(3.29U/mL)، درجة حرارة 28C (3.15 U/mL) وpH=5.0 (3.86 U/mL). وتبين من المقارنة بين متوسطات الفعالية الأنزيمية لكلا الفطرين تفوّق الفطر T.harzianum بإنتاجه للسيلّولاز على الفطر A.alternate، كما وتبين أنّ تأثير زمن الحضن على إنتاج السيلّولاز من الفطر T.harzianum أكبر من تأثيره على إنتاجه من الفطر A.alternate، في حين تأثير كل من درجة الحرارة ودرجة pH على إنتاج السيلّولاز من الفطر T.harzianum أقل من تأثيرهما على إنتاجه من الفطر A.alternate. It was detected for the ability of a local fungus T.harzianum and A.alternate which was isolated from the rhizosphere of Wheat plant Triticum aestivum L. to produce the enzyme complex cellulase in solid medium (CMC-Agar). So it has been showed that T.harzianum and A.alternate have an ability to produce this enzyme, While the T.harzianum have higher production of cellulase than A.alternate. Then conducted a quantitative test using Mandelium liquid medium is used to determine the optimal conditions (incubation time, temperature, PH) for the best cellulase production of the two fungus studied. It was, the best conditions for its production, from T.harzianum is 7 days inchubation (3.97 U/mL), temperature 26C (4.07 U/mL) and pH=5.5 (4.19U/mL). While the best conditions for its production from A.alternate is 8 days inchubation (3.29U/mL), temperature 28C (3.15 U/mL) and pH=5.0 (3.86 U/mL). The comparison between the enzymatic activity average for both fungus , was showed superiority T.harzianum by production cellulase than fungus A.alternate. And discern that the effect of the incubation time on cellulase production from fungus T.harzianum more than its effect on cellulase production from fungus A.alternate. While effect of pH and temperature on cellulase production from fungus T.harzianum less than their effect on cellulase production from fungus A.alternate.

Downloads

Published

2016-10-23

How to Cite

سارة س. (2016). مقارنة بين الظروف المثلى لإنتاج أنزيم السيلّولاز من العزلتين الفطريتين المحليتينT.harzianum و A. alternate. Tishreen University Journal -Biological Sciences Series, 37(4). Retrieved from https://journal.tishreen.edu.sy/index.php/bioscnc/article/view/2095

Issue

Vol. 37 No. 4 (2015): العلوم البيولوجية

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

The authors retain the copyright and grant the right to publish in the magazine for the first time with the transfer of the commercial right to the Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series   

 Under a CC BY- NC-SA 04 license that allows others to share the work with of the work's authorship and initial publication in this journal. Authors can use a copy of their articles in their scientific activity, and on their scientific websites, provided that the place of publication is indicted in Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series  .  The Readers have the right to send, print and subscribe to the initial version of the article, and the title of Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series    Publisher

journal uses a CC BY-NC-SA license which mean

You are free to:

  • Adapt — remix, transform, and build upon the material
  •  
  • The licensor cannot revoke these freedoms as long as you follow the license terms.

 

  • Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • NonCommercial — You may not use the material for commercial purposes.
  • ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
  • No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.