اختبار سمية عزلات فطرية من عينات بذور وكسبة فول الصويا تابعة لأنواع Aspergillus spp.، Penicillium spp. باستخدام بعض الاختبارات الحيوية

Authors

  • صباح المغربي
  • محمود حسن
  • نهى عليو

Abstract

تم اختبار سمية /116/ عزلة فطرية نقية تابعة لأنواع Aspergillus spp. و Penicillium spp. على إنبات بذور الرشاد (Ls) Lepidium sativum، نمو سلالة من البكتريا Bacillus subtilis (Bs) FZB27، وفطر الخميرة (Sc) Saccharomyces cerevisiae، وذلك على ثلاثة مستنبتات غذائية: تشابك آجار CA، خلاصة المالت والخميرة والآجار MYA وآجار الصويا والتريبتون TSA.

أظهر اختبار Ls سمية العزلات بشكل كبير حيث منعت 69، 61 و56 عزلة على المستنبتات
TSA، CA، MYA على التوالي إنبات بذور الرشاد، وكان عدد العزلات السامة في اختباري Bs وSc منخفضاً مقارنة بالاختبار السابق (7، 17، و14 في Bs، و6، 7 و6 في Sc) وذلك على المستنبتات السابقة نفسها وبالترتيب ذاته، وبدت سمية العزلات واضحة على مستنبت TSA في اختباري Ls وSc تلاه مستنبتي CA وMYA، في حين كانت السمية أعلى على مستنبت CA في اختبار Bs تلاه مستنبت TSA ومن ثم MYA، ولوحظ منع النمو بشكل رئيسي في التركيز الأعلى (20 ميكروليتر) في الاختبارات الثلاثة وعلى كل المستنبتات.

The toxicity of /116/ pure fungal isolates belonging to Aspergillus spp. and Penicillim spp. was tested on the germination of garden cress Lepidium sativum (Ls) seeds, the growth of bacterial strain Bacillus subtilis (Bs) FZB27, and the yeast fungus Saccharomyces cerevisiae (Sc), using three media CA, MYA, and TSA.

The toxicity of isolates was evident with the Ls test, in which 69, 61, 56 toxic isolates on TSA, CA, MYA consecutively inhibited the germination of garden cress seeds. The number of toxic isolates with the Bs and Sc tests was low compared to Ls (7, 17, 14 for Bs and 6, 7, 6 for Sc) consecutively on the previous media. The toxicity of isolates was clear on TSA medium with Ls and Sc tests followed by CA and MYA, while it was higher on CA medium with Bs test followed by TSA and MYA. The inhibition of growth was observed at the higher concentration (20 Micro liter) using the three tests and on all media

Downloads

Published

2019-01-09

How to Cite

المغربي ص., حسن م., & عليو ن. (2019). اختبار سمية عزلات فطرية من عينات بذور وكسبة فول الصويا تابعة لأنواع Aspergillus spp.، Penicillium spp. باستخدام بعض الاختبارات الحيوية. Tishreen University Journal -Biological Sciences Series, 31(4). Retrieved from https://journal.tishreen.edu.sy/index.php/bioscnc/article/view/6038

Issue

Vol. 31 No. 4 (2009): العلوم البيولوجية

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

The authors retain the copyright and grant the right to publish in the magazine for the first time with the transfer of the commercial right to the Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series   

 Under a CC BY- NC-SA 04 license that allows others to share the work with of the work's authorship and initial publication in this journal. Authors can use a copy of their articles in their scientific activity, and on their scientific websites, provided that the place of publication is indicted in Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series  .  The Readers have the right to send, print and subscribe to the initial version of the article, and the title of Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series    Publisher

journal uses a CC BY-NC-SA license which mean

You are free to:

  • Adapt — remix, transform, and build upon the material
  •  
  • The licensor cannot revoke these freedoms as long as you follow the license terms.

 

  • Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • NonCommercial — You may not use the material for commercial purposes.
  • ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
  • No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Most read articles by the same author(s)

<< < 1 2 3 4 > >>